enzyme amplification
英 [ˈenzaɪm ˌæmplɪfɪˈkeɪʃn]
美 [ˈenzaɪm ˌæmplɪfɪˈkeɪʃn]
网络 酶增殖
双语例句
- Methods Based on the principle of dehydrogenase coenzyme system, sodium pyruvate was used as a lactate dehydrogenase ( LDH) blocker, NAD+-NBT-Brij 98 was composed to enzyme coupling amplification technique in Tris HCl buffer.
方法基于脱氢酶辅酶体系的原理，用丙酮酸钠作乳酸脱氢酶（LDH）的阻止剂，在TrisHCl缓冲溶液中，由NAD+-NBT-Brij98组成酶偶联放大系统。 - These factors included: the concentrations of Taq polymerase enzyme, MgCl 2, template, dNTPs, primer, amplification of paired primers and thermal cycles.
这些因素包括：Taq酶、MgCl2、模板、dNTPs、引物浓度，双引物扩增及热循环数。 - Objective To research on the possibility and the efficiency of the enzyme biological signal amplification system applied to DNA piezoelectric biosensor.
目的研究酶生物信号放大系统用于放大DNA压电传感器检测信号的可行性和有效性。 - DNA sequencing, restriction enzyme digestion and PCR amplification all confirmed the inserted fragment was canine IL-2 and its sequence was identical to that published in GenBank.
分离纯化扩增片段，克隆入pMD18-T载体，经酶切鉴定、PCR鉴定及DNA序列测序，结果表明扩增片段为犬白细胞介素-2基因，该基因与GenBank上发表的序列一致。 - Results For LCN template DNA, if only increased the amount of Taq enzyme or the reaction volume, the results of STR amplification were scarcely improved, but if increased PCR cycles, the detection sensitivity were significantly improved.
结果对低拷贝模板DNA，单纯增加Taq酶量或反应体系，扩增效率改善不明显；增加循环数，显著提高检验灵敏度； - Research on applying enzyme biological signal amplification system to DNA piezoelectric biosensor
应用酶生物信号放大系统放大DNA压电传感器检测信号的研究 - A new enzyme amplification system which can be widely used in ELISA was developed.
建立了一种能广泛用于酶标免疫吸附分析的新酶放大系统。 - A New Enzyme Amplification System and Its Application
一种新酶放大系统及其应用 - The result indicates that the ( amplification) capability of Tgo enzyme is the same as that of Taq enzyme. But the amplification fidelity of Tgo enzyme is over 30 times higher than that of Taq enzyme and 1.6 times higher than that of Pfu.
该酶的扩增性能与Taq酶相当，而扩增忠实性分别比Taq酶、Pfu酶高30倍和1.6倍。 - Determination of total bile acids in serum with an enzyme coupling amplification technique
酶偶联放大法测定血清总胆汁酸 - This assay showed high sensitivity by taking advantage of sample enrichment and enzyme amplification, and exhibited good specificity by involving the selective aptamer binding and the specific enzyme reaction.
利用样品富集和酶催化放大，实现了对凝血酶的高灵敏检测，而适配体的选择性结合和酶的特异性反应使方法具有高特异性。 - Because of special mechanisms of enzyme effects, a single enzyme molecule is potentially capable of cleaving many prodrug molecules, providing an amplification effect giving high level of active drugs localized on tumors.
由于酶分子作用机制的特殊性，单一酶分子具有水解多个前药分子的潜能，提供了一种放大作用，使活性药物以高浓度定位于肿瘤局部。 - The major results are as follows: 1. Compare the different effects of the dosage of template DNA, Mg2+, dNTPs and Taq enzyme in PCR amplification, then establish an ideal PCR reaction system to test cucumber hybrids purity. 2.
主要研究结果如下：1.比较模板DNA、Mg2+、dNTPs和Taq酶的不同用量对PCR扩增的影响，建立了适用于黄瓜杂种纯度检测的理想PCR反应体系。 - If the system under test contains a target DNA, the DNA probe of the hairpin turn and the subsequent enzyme is not their role, and can be combined with nano-gold-labeled detection probe making signal amplification.
如果待测体系中含有目标DNA，则DNA探针的发夹结构打开且随后的内切酶对其没有作用，并可以结合纳米金标记的检测探针而使信号放大。 - The high temperatures required for PCR necessitate a thermostable enzyme for DNA amplification.
PCR反应扩增DNA是在高温条件下进行的，因此需要一种热稳定的酶。 - Through optimization of the experimental steps including restriction enzyme cutting, ligation, pre-amplification and amplification, an optimized cDNA-AFLP system of cucumber in vitro gynogenesis at early stage was established which showed a distinct electrophoretogram. 3.
通过优化酶切、连接、预扩增和选择性扩增等环节，建立了适宜黄瓜离体雌核发育早期特异基因表达研究的cDNA-AFLP分析体系，并得到了较为清晰可辨的cDNA-AFLP指纹图谱。 - After the Verification by enzyme digestion and PCR amplification, we believe it is the target recombinant genes, and then we sent it to the company to confirmed the sequencing. 3.
经过酶切和PCR扩增验证其为预期的目的基因，送由公司测序确认。